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Category:	Science
Word Count:	578
Featured Papers from Direct Essays 1. Resistance Is Futile: The Dile

	Isolation of Large Plasmids
	Isolation of Large Plasmids from Gram-Negative Bacteria Goal: We suspect the antibiotic “X” resistance gene is located on large plasmids in P. aeruginosa. We need to isolate bacterial plasmids for further analysis. Cell Growth: 1) Select one of the P. aeruginosa colonies that grew on the antibiotic “X” (+) medium, and use this to inoculate 5ml of TSB medium in at 18 X 150mm test tube. Grow the culture overnight (16-18 hours) at 30◦C using a tube roller set at 100-200 rpm.

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Isolation of Large Plasmids