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Category:	Science
Word Count:	310
Featured Papers from Direct Essays 1. Chemistry in depth 2. Resistance Is Futile: The Dile 3. Cloning

	isolation of plasmid dna
	Plasmid DNA was isolated from single colony of E.coli by alkaline lysis method. Alkaline lysis is used to isolate closed circular plasmid DNA from bacteria prior to plasmid purification. Single colony of E.coli strain DH5Ľá containing non-directional cloning vector, pTrcHis2 was inoculated in LB medium containing antibiotic ampicilin. E.coli was cultured overnight to let them replicate the plasmid and genome and become amplified then transferred into two microcentrifuge tubes. Milky white soluti

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isolation of plasmid dna